Reading a BPC-157 and TB-500 Blend Certificate

BPC-157 and TB-500 are frequently sold together in one vial, and a blend certificate works differently from a single-compound one. The most important difference: a blend has no single purity figure, and any vendor quoting one is either misreading the report or hoping you will.

What the two compounds are, analytically

• BPC-157 is a 15-residue peptide, unmodified and water-soluble, with a free N-terminus. Nothing unusual in the chromatography.
• TB-500 is the common name for a fragment of thymosin beta-4, usually the short active-site sequence rather than the full 43-residue protein. The expected mass depends on which molecule was actually supplied, and “TB-500” is a trade name rather than a defined chemical entity.

That second point is where blend reports most often go wrong. If the mass result corresponds to the short fragment while the product is sold as full-length thymosin beta-4, or the reverse, the material is not what the label implies.

Why a blend cannot have one purity number

Purity is the main peak’s share of total integrated peak area. In a blend there are two main peaks by design. Integrating either against the total gives a number near 50%, which describes the mixing ratio rather than cleanliness.

What a blend report can give you instead:

• Content per component, in milligrams per vial, each quantified against that compound’s own reference standard.
• Identity per component, by mass spectrometry.
• Impurity information from the chromatogram, read by eye rather than as a single percentage.

Our guide on how blends are tested covers the laboratory side.

Reading the content figures

Check the ratio, not just the total. A 20 mg blend reporting 12 mg of one component and 8 mg of the other still totals 20 mg, but the vial does not contain what the product page describes. Where three vials were tested, compare the ratio across all three: consistent ratios indicate the components were mixed properly before filling, scattered ratios indicate they were not.

Five questions for a blend certificate

1. Is there a separate content figure for each component, or one combined number?
2. Does the report name the exact TB-500 species tested, with a mass that matches it?
3. Was each component quantified against its own reference standard?
4. Is a single purity percentage quoted for the blend? If so, the vendor has misread its own report.
5. Does the lot number match the vial, and does the report resolve at the laboratory? See verifying a report.

Our blend lots

BB20 (BPC-157 + TB-500) and KLOW (GHK-Cu, TB-500, BPC-157, KPV) are published with a content figure per component, exactly as the laboratory reported them, on each product page and on the lab results page. Neither carries a single purity percentage, because the analysis does not produce one.

Materials supplied by BMS Labs are for laboratory research use only and are not for human or veterinary use. This page describes analytical documentation only and makes no claims about biological effects.